what is hplc used for Fundamentals Explained
The focus of polynuclear aromatic hydrocarbons (PAH) in soil are determined by 1st extracting the PAHs with methylene chloride. The extract is diluted, if needed, plus the PAHs separated by HPLC utilizing a UV/Vis or fluorescence detector. Calibration is obtained utilizing one or more external specifications. In a typical analysis a 2.013-g sample